Abstract: In this paper, the whole-tailings from Xiaodonggou Gold Mine as filling slurry were studied by measuring its rheological parameters using a Brookfield RSR-SST rheometer. The experimental results showed that the rheological curve of whole-tailings filling slurry in the mine fitted to H-B rheological model. With the increase of concentration, the yield stress showed a typical exponential function relationship. When the concentration exceeded 72%, the yield stress and plastic viscosity increased significantly. Through the uniaxial compressive strength test of whole-tailings under different concentrations and ash sand ratios of 3, 7, 14 d, the relationship between the strength of filling body and the ratio parameters of filling materials was obtained. The microstructure of hydration products in the filling body was analyzed by SEM, and the evolution law of hydration products in the filling body with the increase of ash sand ratio was obtained, providing a theoretical basis for realizing high concentration filling of whole-tailings in Xiaodonggou Gold Mine.